PROTEIN FLUORESCENCE by Joseph R. Lacowicz (Editor) ( 9781475781946 )

Item Details

Title:

PROTEIN FLUORESCENCE

By:

Format:

Paperback

List price:	£159.99
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ISBN 10:

1475781946

ISBN 13:

9781475781946

Publisher:

SPRINGER-VERLAG NEW YORK INC.

Pub. date:

1 July, 2013

Edition:

Softcover reprint of the original 1st ed. 2002

Series:

Topics in Fluorescence Spectroscopy 6

Pages:

310

Synopsis:

The intrinsic or natural fluorescence of proteins is perhaps the most complex area of biochemical fluorescence. Fortunately the fluorescent amino acids, phenylalanine, tyrosine and tryptophan are relatively rare in proteins. Tr- tophan is the dominant intrinsic fluorophore and is present at about one mole % in protein. As a result most proteins contain several tryptophan residues and even more tyrosine residues. The emission of each residue is affected by several excited state processes including spectral relaxation, proton loss for tyrosine, rotational motions and the presence of nearby quenching groups on the protein. Additionally, the tyrosine and tryptophan residues can interact with each other by resonance energy transfer (RET) decreasing the tyrosine emission. In this sense a protein is similar to a three-particle or mul- particle problem in quantum mechanics where the interaction between particles precludes an exact description of the system. In comparison, it has been easier to interpret the fluorescence data from labeled proteins because the fluorophore density and locations could be controlled so the probes did not interact with each other. From the origins of biochemical fluorescence in the 1950s with Prof- sor G. Weber until the mid-1980s, intrinsic protein fluorescence was more qualitative than quantitative. An early report in 1976 by A. Grindvald and I. Z. Steinberg described protein intensity decays to be multi-exponential. Attempts to resolve these decays into the contributions of individual tryp- phan residues were mostly unsuccessful due to the difficulties in resolving closely spaced lifetimes.

Illustrations:

XXI, 310 p.

Publication:

Imprint:

Springer-Verlag New York Inc.

Returns:

Returnable

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PROTEIN FLUORESCENCE (HB)