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Item Details
| Title:
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A LABORATORY GUIDE TO GENOMIC SEQUENCING
THE DIRECT SEQUENCING OF NATIVE UNCLONED DNA |
| By: |
H. P. Saluz, Jean Pierre Jost |
| Format: |
Paperback |
| List price:
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£72.00 |
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We currently do not stock this item, please contact the publisher directly for
further information.
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| ISBN 10: |
3764319259 |
| ISBN 13: |
9783764319250 |
| Publisher: |
BIRKHAUSER VERLAG AG |
| Pub. date: |
1 January, 1987 |
| Series: |
Biomethods |
| Pages: |
164 |
| Synopsis: |
A Safety Considerations Genomic sequencing involves a number of hazardous steps, such as high current, high voltage, radioactive and highly toxic chemicals. It is, therefore, absolutelyessen- tial that the instructions of equipment manufacturers be followed and that particular attention is paid to the local and federal safety regulations. INTRODUCTION 9 B Introduction During the cloning of genomic DNA many of its characteristics are perma- nently lost. It was therefore necessary to develop a new technique that would give us a closer look at a gene in its normal environment. The powerful technique of genomic sequencing, first described by Church and Gilbert (1984) now makes it possible to have a precise view of a given DNA sequence in a chromosome. This method combines the chemical DNA-sequencing procedure of Maxam and Gilbert (1980) with the detection of DNA sequences by electroblotting and indirect end-labeling by hybridization. Besides studies on the methylation state of single bases in a given gene (Nick et al. , 1986; Saluz and Jost, 1986; Saluz et al. , 1986), genomic sequencing can also be used to study specific DNA-protein interactions in vivo (Church et al., 1985; Giniger et al. , 1985; Becker et al. , 1986; Ephrussi et al. , 1985; Martin et al. , 1986; Nick et al. , 1986; Zinn and Maniatis, 1986). |
| Illustrations: |
31 Illustrations, black and white; 164 p. 31 illus. |
| Publication: |
Switzerland |
| Imprint: |
Birkhauser Verlag AG |
| Returns: |
Returnable |
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