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Item Details
Title:
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LIPID BIOCHEMISTRY
AN INTRODUCTION |
By: |
Dr. Michael I. Gurr, Anthony Trafford James, John L. Harwood |
Format: |
Paperback |
List price:
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£69.99 |
We currently do not stock this item, please contact the publisher directly for
further information.
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ISBN 10: |
0412266202 |
ISBN 13: |
9780412266201 |
Publisher: |
CHAPMAN AND HALL |
Pub. date: |
28 February, 1991 |
Edition: |
4th Revised edition |
Pages: |
406 |
Description: |
A discussion of the biochemistry of lipids, including classification, structure and metabolism, implications for health and disease and the functions of lipids. The book is intended for undergraduates, but may be a useful first reference for postgraduates and research workers. |
Synopsis: |
Lipids can usually be extracted easily from tissues by making use of their hydrophobic characteristics. However, such extractions yield a complex mixture of different lipid classes which have to be purified further for quantitative analysis. Moreover, the crude lipid extract will be contami- nated by other hydrophobic molecules, e.g. by intrinsic membrane proteins. Of the various types of separation processes, thin layer and column chromatography are most useful for intact lipids. High performance liquid chromatography (HPLC) is also rapidly becoming more popular, especially for the fractionation of molecular species of a given lipid class. The most powerful tool for quantitation of the majority of lipids is gas- liquid chromatography (GLC). The method is very sensitive and, if adapted with capillary columns, can provide information with regard to such subtle features as the position or configuration of substitutions along acyl chains. By coupling GLC or HPLC to a radioactivity detector, then the techniques are also very useful for metabolic measurements.Although research laboratories use generally sophisticated analytical methods such as GLC to analyse and quantify lipid samples, chemical derivatie:ations are often used in hospitals. For these methods, the lipid samples are derivatized to yield a product which can be measured simply and accurately-usually by colour. Thus, total triacylglycerol, cholesterol or phospholipid-phosphorus can be quantitated conveniently without bothering with the extra information of molecular species, etc. which might be determined by more thorough analyses. REFERENCES Christie, w.w. (1982) Lipid Analysis, 2nd edn, Pergamon Press, Oxford. |
Illustrations: |
32 black & white illustrations, biography |
Publication: |
UK |
Imprint: |
Chapman and Hall |
Returns: |
Non-returnable |
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