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Item Details
| Title:
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NUCLEIC ACID AMPLIFICATION TECHNOLOGIES: APPLICATION TO DISEASE DIAGNOSIS
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| By: |
Olsvik (Editor), Morse (Editor), Lee (Editor) |
| Format: |
Hardback |
| List price:
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£72.00 |
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We currently do not stock this item, please contact the publisher directly for
further information.
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| ISBN 10: |
0817639217 |
| ISBN 13: |
9780817639211 |
| Publisher: |
BIRKHAUSER BOSTON INC |
| Pub. date: |
15 July, 1997 |
| Edition: |
1996 ed. |
| Pages: |
286 |
| Description: |
Providing current information and guidance on the uses of various nucleic acid amplification technologies for clinical laboratory diagnosis, this volume goes beyond the Polymerase Chain Reaction to explore a broader range of important alternative DNA/RNA amplification methods. |
| Synopsis: |
The polymerase chain reaction (PCR) has proved to be a powerful and versatile tool and has opened new avenues in molecular biol- ogy. Alternative nucleic acid amplification techniques, such as the ligase chain reaction (LCR), nucleic acid sequence-based amplifica- tion (NASBA), and transcription-mediated amplification (TMA), a variation of NASBA, are also now available. These techniques are all designed to amplify specific nucleic acid sequences in an expo- nential manner, thus providing a basis for extremely sensitive diag- nostic assays. However, despite the widespread and successful ap- plication of genomic amplification techniques in biological research, they have not yet reached the point of routine use in clini- cal laboratories. Thus, although the R&D investment in nucleic acid diagnostics is in excess of $250 million annually, clinical ap- plications remain relatively modest. One of the principal reasons for this delay in clinical application has been the problem of acci- dental contamination of negative clinical specimens with minute amounts of amplified products from a previous positive reaction. Carry-over contamination of amplicons can now be prevented by chemical means or the use of a closed reaction system. However, the current instrumentation is essentially modular in nature, com- prising machines that perform the three essential steps of nucleic acid amplification technology: sample preparation, the amplifica- tion reaction, and detection of products. Consequently, the test pro- cedures are more complicated with somewhat lower sample throughput than the enzyme immunoassays currently performed in clinical laboratories. |
| Illustrations: |
X, 286 p. |
| Publication: |
US |
| Imprint: |
Birkhauser Boston Inc |
| Returns: |
Returnable |
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