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Item Details
| Title:
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MONOCLONAL ANTIBODY PROTOCOLS
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| By: |
William C. Davis |
| Format: |
Paperback |
| List price:
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£105.50 |
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We currently do not stock this item, please contact the publisher directly for
further information.
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| ISBN 10: |
0896033082 |
| ISBN 13: |
9780896033085 |
| Publisher: |
HUMANA PRESS INC. |
| Pub. date: |
23 May, 1995 |
| Edition: |
1995 ed. |
| Series: |
Methods in Molecular Biology v. 45 |
| Pages: |
264 |
| Description: |
This text provides a set of methods for producing and using monoclonal antibodies in biomedical, agricultural and biological sciences. |
| Synopsis: |
Since the initial description of techniques to immortalize anti- body-producing B-lymphocytes by fusion with tissue culture-adapted myeloma cells, methods have been developed to produce monoclonal antibodies of defined specificity in multiple animal species. Stable hybrids can be readily produced in mice using a number of myeloma and hybridoma cell lines. To obviate the problem of identifying fusion partners in other animal species, xenohybrids have been produced using B-lymphocytes from the relevant species and mouse myeloma cells. The use of xenohybrids has minimized the problem of obtain- ing stable antibody-producing hybrids in all species examined thus far. Although alternative techniques are being developed to produce monoclonal antibodies by molecular methods, hybridoma technol- ogy will remain the technology of choice for producing monoclonal antibodies for a variety of applications in research and industry. The objective of Monoclonal Antibody Protocols is to provide investigators with a set of methods for producing and using mono- clonal antibodies in biomedical, agricultural, and biological sciences.The book is not intended to provide methodology for all possible applications, but rather a series of methods presented in an easy-- follow format that can be used by new and established investiga- tors, graduate and postgraduate fellows, and technical staff. |
| Illustrations: |
biography |
| Publication: |
US |
| Imprint: |
Humana Press Inc. |
| Returns: |
Returnable |
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