PATCH-CLAMP APPLICATIONS AND PROTOCOLS by Alan A. Boulton (Editor), Glen B. Baker (Editor), Wolfgang Walz (Editor) ( 9780896033115 )

Item Details

Title:

PATCH-CLAMP APPLICATIONS AND PROTOCOLS

By:

Alan A. Boulton (Editor), Glen B. Baker (Editor), Wolfgang Walz (Editor)

Format:

Hardback

List price:	£129.99
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ISBN 10:

0896033112

ISBN 13:

9780896033115

Publisher:

HUMANA PRESS INC.

Pub. date:

1 May, 1995

Series:

Neuromethods v. 26

Pages:

316

Description:

Covers the techniques for measuring and recording directly activity within a cell. This book offers developments associated with the technology of path-clamp electrodes, of cell-free ion-channel recording, and of the whole-cell patch clamp technique. It also covers the loose patch-clamp technique, single-channel recording, and more.

Synopsis:

E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby decreasing background membrane noise. In order to reduce the dominant source of background noise-the leakage shunt under the pipet rim between m- the muscle membrane had to be treated brane and gla- enzymatically. Despite these early limitations, a new te- nique was born -the patch-clamp technique. The final bre- through came in 1981 when the same authors, in collaboration with 0. P. Hamill, A. Marty, and F. J. Sigworth, developed the gigaohm seal. Not only did this improve the quality of recordings, it was now possible to gently pull the membrane patch with the attached pipet off the cell and study its trapped ion channels in isolation. Another offshoot of the gigaohm seal technique was the whole-cell patch-clamp technique, in which the patch is ruptured without breaking the seal.This technique is really a sophisticated voltage-clamp technique and also allows for the altering of cytoplasmic constituents if the experimenter so wishes. The first part of Patch-Clamp Applications and Protocols presents modern developments associated with the techn- ogy of patch-clamp electrodes, of cell-free ion channel reco- ing, and of the whole-cell patch-clamp technique.

Illustrations:

biography

Publication:

Imprint:

Humana Press Inc.

Returns:

Returnable

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