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Item Details
Title:
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CAPILLARY ELECTROPHORESIS OF NUCLEIC ACIDS
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Volume: |
v. 2 |
By: |
Keith R. Mitchelson (Editor), Jing Cheng (Editor) |
Format: |
Hardback |

List price:
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£129.99 |
We currently do not stock this item, please contact the publisher directly for
further information.
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ISBN 10: |
0896037657 |
ISBN 13: |
9780896037656 |
Publisher: |
HUMANA PRESS INC. |
Pub. date: |
19 January, 2001 |
Edition: |
2001 ed. |
Series: |
Methods in Molecular Biology v. 163 |
Pages: |
408 |
Description: |
Capillary electrophoresis (CE) provides researchers with the ability to separate and analyze very small amounts of DNA. This second volume presents practical considerations necessary for very rapid and accurate separations of linear DNAs by CE using short capillaries. |
Synopsis: |
The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures to utilize this new resource for analysis of genetic variation and for the detection of disease causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and analyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analysis are manifold. Further, the high sensitivity of detection, and the ab- ity to increase sample throughput with parallel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA-adducts and single-strand oligonucleotides through to PCR-amplified DNA fragments and whole chromosomes. Capillary Electrophoresis of Nucleic Acids focuses on such analytical protocols, which can be used for detection and analysis of mutations and modification, from precise DNA loci through to entire genomes of organisms.Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications. |
Illustrations: |
biography |
Publication: |
US |
Imprint: |
Humana Press Inc. |
Returns: |
Returnable |
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