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Item Details
| Title:
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IMMUNOCHEMICAL PROTOCOLS
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| By: |
Robert Burns (Editor) |
| Format: |
Paperback |
| List price:
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£127.50 |
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We currently do not stock this item, please contact the publisher directly for
further information.
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| ISBN 10: |
1617374660 |
| ISBN 13: |
9781617374661 |
| Publisher: |
HUMANA PRESS INC. |
| Pub. date: |
28 October, 2010 |
| Edition: |
Softcover reprint of hardcover 3rd ed. 2005 |
| Series: |
Methods in Molecular Biology 295 |
| Pages: |
318 |
| Description: |
The basis for the science is the exquisite elegance of the mammalian immune system with its ability to recognize foreign proteins and to manufacture antibody m- ecules that strongly bind to the substances that elicited them. |
| Synopsis: |
Immunochemical techniques have been in use for many years with early examples of bacterial strain typing dating back to the 1940s. The basis for the science is the exquisite elegance of the mammalian immune system with its ability to recognize foreign proteins and to manufacture antibody m- ecules that strongly bind to the substances that elicited them. Not only are potentially harmful pathogens and toxins recognized by the immune system, but the system can be persuaded to manufacture antibodies to an astonishing array of substances. In the early days of this science, all antibodies for investigative work were produced by immunizing mammals with the substance of interest, followed by regular donor bleeds that yielded antisera. Serum produced in this way yields heterogenic populations of antibody molecules recognizing different epitopes on the target protein, which may be adequate for its intended p- poses, but can also cause problems of crossreactivity. In 1975, Kohler and Milstein reported that spleen cells from immune donor animals could be immortalized, cloned from single cells, and grown in continuous culture. This original work described the method for the production of monoclonal antibodies. |
| Illustrations: |
22 Illustrations, black and white; X, 318 p. 22 illus. |
| Publication: |
US |
| Imprint: |
Humana Press Inc. |
| Returns: |
Returnable |
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