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Item Details
| Title:
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ENZYME ENGINEERING
METHODS AND PROTOCOLS |
| By: |
James C. Samuelson (Editor) |
| Format: |
Hardback |
| List price:
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£139.99 |
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We currently do not stock this item, please contact the publisher directly for
further information.
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| ISBN 10: |
1627032924 |
| ISBN 13: |
9781627032926 |
| Publisher: |
HUMANA PRESS INC. |
| Pub. date: |
20 February, 2013 |
| Edition: |
2013 ed. |
| Series: |
Methods in Molecular Biology 978 |
| Pages: |
252 |
| Description: |
This book provides guidance to those wishing to create enzyme variants. It covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes and the engineering of two difference types of rare-cutting endonucleases. |
| Synopsis: |
Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. Enzyme Engineering: Methods and Protocols provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible Enzyme Engineering: Methods and Protocols will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs. |
| Illustrations: |
22 Tables, black and white; XI, 252 p. 52 illus., 19 illus. in color. |
| Publication: |
US |
| Imprint: |
Humana Press Inc. |
| Returns: |
Returnable |
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