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Item Details
Title: EXPRESSION SYSTEMS
METHODS EXPRESS
By: Michael Dyson (Editor), Yves Durocher (Editor)
Format: Hardback

List price: £70.00
Our price: £66.50
Discount:
5% off
You save: £3.50
ISBN 10: 1904842453
ISBN 13: 9781904842453
Availability: Reprinting. This item may be subject to delays or cancellation.
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Stock: Currently 0 available
Publisher: SCION PUBLISHING LTD
Pub. date: 15 September, 2007
Series: Methods Express Series
Pages: 288
Description: Protein expression is an increasingly important tool for research on gene function. Expression Systems: Methods Express is a lab manual providing established methods as well as the latest state-of-the-art protocols, and also clear advice on what expression system to choose when.
Synopsis: Protein expression is an increasingly important tool for research on gene function. What is needed is not just a lab manual providing established methods as well as the latest state-of-the-art protocols, but also clear advice on what expression system to choose when. Expression Systems: Methods Express uniquely fills this need. It covers expression across a broad range of systems, including the following. *Baculovirus expression vectors*CHO cells* E. coli*HEK293-EBNA1 cells* Lactococcus lactis and other gram positive bacteria* S. cerevisiae*transfected insect cells* Pichia pastoris*mammalian cells using BacMam viruses*lentiviral vectors*wheat germ cell-free systemThe book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. It also provides step-by-step methods for each system. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression. Every chapter discusses the merits and limitations of the approaches available, describes the key techniques in full practical detail, and provides sensible advice for immediate use at the bench. In summary, Expression Systems: Methods Express is a comprehensive laboratory manual and information resource for researchers at all levels, from postgraduate student to principal investigator.
Publication: UK
Imprint: Scion Publishing Ltd
Returns: Returnable
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