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Item Details
| Title:
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PCR SEQUENCING PROTOCOLS
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| By: |
Ralph Rapley |
| Format: |
Book |
| List price:
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£89.99 |
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We currently do not stock this item, please contact the publisher directly for
further information.
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| ISBN 10: |
0896033449 |
| ISBN 13: |
9780896033443 |
| Publisher: |
HUMANA PRESS INC. |
| Pub. date: |
19 August, 1996 |
| Series: |
Methods in Molecular Biology 65 |
| Pages: |
221 |
| Description: |
Offers readers a collection of the most reliable protocols for PCR sequencing - all developed and described by researchers and scientists who use them successfully every day. |
| Synopsis: |
Advances in bioscience research usually arise as a result of the continu- ing refinement of existing technologies. However, there are a number of occa- sions v^rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegantly simple in concept and require minimal technical manipulation. Two of these revolu- tionary techniques are the focus ofPCR Sequencing Protocols. The first such technique is enzymatic chain termination sequencing developed by Sanger and his co-workers in Cambridge and reported in 1977. This essentially brought the possibility of deriving nucleotide sequence information in a very short time scale and has been widely accepted in many laboratories as a routine molecular biological research tool. Furthermore, it has not only led to the sequencing of many genes and gene fragments, but has also allowed the tech- nical means of sequencing the human genome. The second technique that has found widespread acceptance in basic applied research and many routine applications is the polymerase chain reac- tion.This technique, first reported in 1985 by MuUis and his colleagues, pro- vides the means to amplify nucleic acid sequence, which immediately proved invaluable in nearly all fields of biological laboratory research. Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase. |
| Illustrations: |
24 black & white illustrations, biography |
| Publication: |
US |
| Imprint: |
Humana Press Inc. |
| Returns: |
Returnable |
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