 |
|
|
Item Details
| Title:
|
PCR SEQUENCING PROTOCOLS
|
| By: |
Ralph Rapley (Editor) |
| Format: |
Paperback |
| List price:
|
£109.99 |
|
We currently do not stock this item, please contact the publisher directly for
further information.
|
|
|
|
|
|
| ISBN 10: |
1489940383 |
| ISBN 13: |
9781489940384 |
| Publisher: |
HUMANA PRESS INC. |
| Pub. date: |
13 August, 2013 |
| Edition: |
1996 ed. |
| Series: |
Methods in Molecular Biology 65 |
| Pages: |
221 |
| Synopsis: |
Advances in bioscience research usually arise as a result of the continu- ing refinement of existing technologies. However, there are a number of occa- sions v^rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegantly simple in concept and require minimal technical manipulation. Two of these revolu- tionary techniques are the focus ofPCR Sequencing Protocols. The first such technique is enzymatic chain termination sequencing developed by Sanger and his co-workers in Cambridge and reported in 1977. This essentially brought the possibility of deriving nucleotide sequence information in a very short time scale and has been widely accepted in many laboratories as a routine molecular biological research tool. Furthermore, it has not only led to the sequencing of many genes and gene fragments, but has also allowed the tech- nical means of sequencing the human genome. The second technique that has found widespread acceptance in basic applied research and many routine applications is the polymerase chain reac- tion.This technique, first reported in 1985 by MuUis and his colleagues, pro- vides the means to amplify nucleic acid sequence, which immediately proved invaluable in nearly all fields of biological laboratory research. Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase. |
| Illustrations: |
24 black & white illustrations, biography |
| Publication: |
US |
| Imprint: |
Humana Press Inc. |
| Returns: |
Returnable |
|
|
|
 |
|
|
|
|
|
Little Worried Caterpillar (PB)
Little Green knows she''s about to make a big change - transformingfrom a caterpillar into a beautiful butterfly. Everyone is VERYexcited! But Little Green is VERY worried. What if being a butterflyisn''t as brilliant as everyone says?Join Little Green as she finds her own path ... with just a littlehelp from her friends.
|
|
All the Things We Carry PB
What can you carry?A pebble? A teddy? A bright red balloon? A painting you''ve made?A hope or a dream?This gorgeous, reassuring picture book celebrates all the preciousthings we can carry, from toys and treasures to love and hope. With comforting rhymes and fabulous illustrations, this is a warmhug of a picture book.
|
|
|
|